human hcc cell lines snu387  (Keygen Biotech)

Journal: iScience

Article Title: Targeting Smyd3 by next-generation antisense oligonucleotides suppresses liver tumor growth

doi: 10.1016/j.isci.2021.102473

Figure Lengend Snippet: Smyd3-ASO treatment inhibits proliferation rates and colony formation ability of human hepatocellular carcinoma cell lines (A) Growth curves of HuH7, SNU387, HLF, SNU398, SNU423, and SNU449 cell lines treated with 0.2 μM control-ASO (blue lines) or hSmyd3-ASO-1 (red lines). The cells were pretreated with the ASOs for one passage before plating. Data points represent mean values of the fold increase in cell numbers compared to time 0 (12 hr after seeding) and SEM from 3 independent experiments. (B) Colony formation capacity of HLF, SNU398, SNU423, SNU449, and HuH7 cell lines treated with 0.2 μM control-ASO or hSmyd3-ASO-1 or hSmyd3-ASO-2. The cells were seeded at a density of 1000 cells per 100 mm plate and fixed after 18-22 days. ASO-containing medium was replaced every 3 days. Bars represent the average number of colonies per plate and SEM from 3 experiments. See also .

Article Snippet: The human HCC cell lines SNU387 (from grade IV/V pleomorphic HCC), SNU398, SNU423 (from grade III/IV pleomorphic HCC) and SNU449 (from grade II-IV HCC) were from ATCC, HuH7 and HLF cells were from Japanese Cancer Research Foundation (JCRF).

Techniques: Control

Journal: iScience

Article Title: Targeting Smyd3 by next-generation antisense oligonucleotides suppresses liver tumor growth

doi: 10.1016/j.isci.2021.102473

Figure Lengend Snippet:

Article Snippet: The human HCC cell lines SNU387 (from grade IV/V pleomorphic HCC), SNU398, SNU423 (from grade III/IV pleomorphic HCC) and SNU449 (from grade II-IV HCC) were from ATCC, HuH7 and HLF cells were from Japanese Cancer Research Foundation (JCRF).

Techniques: Recombinant, Protease Inhibitor, Reverse Transcription, SYBR Green Assay, Modification, Software